mTOR Inhibitor XI, Torin1 - Calbiochem

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Highly potent inhibitor for mTOR and DNA-PK while inhibits PI3-K only at much higher concentration	Culture the cells in DMEM medium containing 250nM Torin1

Upstream tips
Highly potent inhibitor for mTOR and DNA-PK while inhibits PI3-K only at much higher concentration
Protocol tips
Culture the cells in DMEM medium containing 250nM Torin1

Publication protocol

For autophagy induction, cells were treated with 250 nM Torin 1 (Sigma–Aldrich), 100 nM and 1 μM Rapamycin (Sigma–Aldrich) or cultured in Earle's Balanced Salt Solution (Sigma–Aldrich).

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