Protocol tips
Upstream tips |
Protocol tips |
Downstream tips |
Intercalates between DNA/RNA and used to analyze mitochondria and Lysosomal content by flow cytometry. |
Stain the cells with 2 μg/ml acridine orange (AO) in phosphate buffered saline for 15 min at 37 °C and immediately analyze with a fluorescence microscope |
|
Upstream tips |
Intercalates between DNA/RNA and used to analyze mitochondria and Lysosomal content by flow cytometry. |
Protocol tips |
Stain the cells with 2 μg/ml acridine orange (AO) in phosphate buffered saline for 15 min at 37 °C and immediately analyze with a fluorescence microscope |
Publication protocol
GL15 cells were grown in glass coverslips for 3 days, then serum starved for 48 hours in the presence or absence of 400 μM LND. The cells were stained with 2 μg/ml acridine orange (AO) in phosphate buffered saline for 15 min at 37 °C and immediately analyzed with a fluorescence microscope (DMRB Leika).
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The energy blockers bromopyruvate and lonidamine lead GL15 glioblastoma cells to death by different p53-dependent routes
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