Acridine Orange hemi(zinc chloride) salt

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Intercalates between DNA/RNA and used to analyze mitochondria and Lysosomal content by flow cytometry.	For acidic vesicular organelle (AVO) staining, incubate cells at 37°C for 15 minutes with acridine orange (1 μg/mL) containing medium. Analyze the cells under FACS.

Upstream tips
Intercalates between DNA/RNA and used to analyze mitochondria and Lysosomal content by flow cytometry.
Protocol tips
For acidic vesicular organelle (AVO) staining, incubate cells at 37°C for 15 minutes with acridine orange (1 μg/mL) containing medium. Analyze the cells under FACS.

Publication protocol

To detect and quantify autophagic activity, cells were plated and irradiated on 60 mm plates under the same conditions as for cell cycle measurement. For acidic vesicular organelle (AVO) staining, cells were incubated at 37°C for 15 minutes with acridine orange (1 μg/mL) containing medium. After trypsinization and washing, pelleted cells were suspended in PBS and subjected to fluorescence-activated cell sorter (FACS) analysis as previously described [14].

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