LC3B Antibody Kit for Autophagy

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	Primary antibody dilution is 1:200

Protocol tips
Primary antibody dilution is 1:200

Publication protocol

Cells were grown on polypropylene film of the microbeam dishes and rinsed with PBS post high LET cytoplasmic irradiation. Cells were fixed with 4% paraformaldehyde in PBS for 15 minutes at room temperature and rinsed three times with PBS. The fixed cells were permeabilized with 0.2% Triton X-100 in PBS for 20 min and stained with 1:200 dilution of the following primary antibodies: γ-H2AX, p-ATM, p-ATR, p-DNA-PKcs and LC3B. Alexa® Fluor 488 or Tetramethyl Rhodamine labeled secondary antibodies were obtained from Jackson Immuno Research (Jackson Immuno Research Labs, INC. West Grove, PA; Cat. 200-542-037 and 200-022-037). DAPI was used for nuclear stain. Images were captured using a laser confocal microscope (Nikon TE200-C1, Japan).

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