DeadEnd™ Colorimetric TUNEL System

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- The slides were treated with 2× SSC buffer, 0.3% hydrogen peroxide and then incubated with streptavidin HPR reaction buffer.

Downstream tips
- The slides were treated with 2× SSC buffer, 0.3% hydrogen peroxide and then incubated with streptavidin HPR reaction buffer.

Publication protocol

Immunostaining, LacZ staining, and H&E staining were performed as described previously (39, 42). Neonatal skeletal muscle was fixed in 4% paraformaldehyde prior to sectioning. A TUNEL assay was performed with a DeadEnd colorimetric TUNEL system (Promega) according to the directions outlined in the user manual. Briefly, the tissue sections were deparaffinized, rehydrated, and permeabilized with protease K buffer before incubation with the TdT reaction mixture. The slides were treated with 2× SSC buffer, 0.3% hydrogen peroxide and then incubated with streptavidin HPR reaction buffer. The sections were imaged with an Zeiss Axio Imager M1 upright microscope equipped with Zen software (39).

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Manufacturer protocol

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