LC3A/B (D3U4C) XP® Rabbit mAb #12741

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	1:100 dilution for immunohistochemistry and 1:1000 dilution for Western blot.

Protocol tips
1:100 dilution for immunohistochemistry and 1:1000 dilution for Western blot.

Publication protocol

HK-2 cells were washed twice in PBS and harvested. Extra-nuclear and intra-nuclear proteins were isolated using a protein extraction kit (Beyotime Institute of Biotechnology), according to the manufacturer's protocol. Western blotting was performed according to a previously published procedure (10). Expression levels of cleaved caspase-3, BiP, CHOP, Nrf2, HO-1, Beclin-1, LC3 A/B, p-mTOR Ser2448, p-mTOR Ser2481, p62 and mTOR were quantified using Image-Pro plus software version 6.0 (Media Cybernetics, Inc., Rockville, MD, USA). Extra-nuclear proteins were normalized to β-actin and intra-nuclear proteins were normalized to lamin B.

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Discussion

Papers

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for LC3A/B (D3U4C) XP® Rabbit mAb #12741 below.

Download manufacturer protocol

Videos

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