APO-BRDU™ Kit (RUO)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- 1.5×10^5 cells were platted in 6cm culture dish	- Cells were fixed in 1% paraformaldehyde in PBS for 30 min. - Dehydrate cells in 70% methanol at 4°C for 30 min.

Upstream tips
- 1.5×10^5 cells were platted in 6cm culture dish
Protocol tips
- Cells were fixed in 1% paraformaldehyde in PBS for 30 min. - Dehydrate cells in 70% methanol at 4°C for 30 min.

Publication protocol

HIG-82 synoviocytes (1.5×105) were plated in 6-cm culture dishes for 24 h. The culture medium was replaced with three types of media: (1) FC medium, (2) MFD medium and (3) FD medium for 48 h. Cells were fixed in 1% paraformaldehyde in PBS for 30 min, then washed with PBS, and stored in 70% methanol at 4°C. After rehydration in PBS, cells were assayed with Apoptosis Detection Kit (APO-BRDU) (BD Pharmigen) [29]. The data were acquired, analyzed and plotted by the CellQuest Pro software and the Sigma Plot 10.0 software.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing TUNEL assay cell type - Rabbit synovial fibroblasts using APO-BRDU™ Kit (RUO) from BD Biosciences.

Manufacturer protocol

Download the product protocol from BD Biosciences for APO-BRDU™ Kit (RUO) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing TUNEL assay cell type - Rabbit synovial fibroblasts using APO-BRDU™ Kit (RUO) from BD Biosciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.