Anti-LC3B antibody (ab48394)

Protocol tips

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	1:1000 dilution of primary antibody

Protocol tips
1:1000 dilution of primary antibody

Publication protocol

After drug treatment for 24 h, QBC939 cells and HepG2 cells were harvested, washed with cold PBS, and incubated in RIPA lysis buffer for 40 min at 4°C to isolate total proteins. The concentration of total proteins was measured using the Bio-Rad assay. Protein samples (30 μg for each group) were separated by 12% SDS-PAGE and transferred to PVDF membranes (Roche, Basel, Switzerland), which were blocked with buffer (100 mM NaCl, 10 mM Tris–HCl [pH7.6], and 0.1% Tween 20) containing 5% non-fat dry milk for 1.5 h at room temperature. Membranes were incubated with various primary antibodies overnight at 4°C and with the corresponding secondary antibodies at room temperature for 1.5 h. The bands were visualized using Pierce ECL Western Blot Substrate (Thermo Scientific, Waltham, MA).

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Manufacturer protocol

Download the product protocol from Abcam for Anti-LC3B antibody (ab48394) below.

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