LC3A/B (D3U4C) XP® Rabbit mAb #12741

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- dilute primary antibody at 1:1000 in 5% w/v BSA, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight.

Protocol tips
- dilute primary antibody at 1:1000 in 5% w/v BSA, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight.

Publication protocol

Western blotting assays were used to examine the expression level of key regulators involved in cell cycle, apoptosis, autophagy, and EMT processes as previously described [61]. Briefly, HT29 and Caco-2 cells were collected and lysed after 48-h treatment with ALS at 0.1, 1, and 5 μM. The supernatant was collected and the protein concentrations were measured using a Pierce™ BCA protein assay kit. Thirty microgram proteins were subjected to Western blotting assay. Visualization was performed using an enhanced chemiluminescence kit (Thermal Scientific, Waltham, MA, USA) and the blots were analyzed using Image Lab 3.0 (BioRad, Hercules, CA, USA). Protein expression level was normalized to the matching densitometric value of the internal control β-actin.

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for LC3A/B (D3U4C) XP® Rabbit mAb #12741 below.

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