Anti-LC3-I/II Antibody

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	-Add primary Ab and incubate with agitation for 1 hour.

Protocol tips
-Add primary Ab and incubate with agitation for 1 hour.

Publication protocol

Immunoblots were performed as described previously.14 Abs to the following proteins were used: actin and CD74 (Santa Cruz Biotechnology); microtubule-associated protein 1 light chain 3 (LC3) and Beclin-1 (Sigma-Aldrich); and p62/SQSTM1 (Medical & Biological Laboratories). Further details are available in supplemental Methods (available on the Blood Web site; see the Supplemental Materials link at the top of the online article).

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Manufacturer protocol

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