Atg5 Antibody

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Incubate the membrane in primary antibody solution for 1 hour at room temperature or overnight at 4˚C with gentle rocking. This time may require optimization. In most cases, overnight incubation at 4˚C increases signal strength and reduces background signal relative to 1 hour incubation at room temperature.

Protocol tips
Incubate the membrane in primary antibody solution for 1 hour at room temperature or overnight at 4˚C with gentle rocking. This time may require optimization. In most cases, overnight incubation at 4˚C increases signal strength and reduces background signal relative to 1 hour incubation at room temperature.

Publication protocol

The STAT3 pathway was detected as previously described (26). Total harvested cells were lysed to perform immunoblots as previously reported (27). Immunoblotting was subjected to semi-quantitative analysis using ImageJ software.

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Reviews

Discussion

Papers

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for Atg5 Antibody below.

Download manufacturer protocol

Videos

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