Dansylcadaverine

Protocol tips

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	If no detectable levels of autophagy in testing samples make sure that Autophagy Reagent A is added 2 hours prior to cell acquisition Label cells with 0.05 mM MDC in PBS at 37°C for 10 min

Protocol tips
If no detectable levels of autophagy in testing samples make sure that Autophagy Reagent A is added 2 hours prior to cell acquisition Label cells with 0.05 mM MDC in PBS at 37°C for 10 min

Publication protocol

Monodansylcadaverine (MDC; Sigma-Aldrich; Merck Millipore, Darmstadt, Germany), a specific marker for autophagic vacuoles was used in order to further confirm whether curcumin was able to induce autophagy. MG63 cells were labeled with 0.05 mM MDC in PBS at 37°C for 10 min, washed three times with PBS and immediately analyzed under an AV300-ASW confocal microscope (Olympus Corp., Tokyo, Japan) with a ×60 oil lens. The amount of LC3 puncta per cell was quantified. A minimum of 10 cells in five independent experiments were analyzed at random.

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Manufacturer protocol

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