Dansylcadaverine

Protocol tips

Upstream tips	Protocol tips	Downstream tips
MDC is a fluorescent compound that stains autophagic vacuoles.	Treat the cells with 0.05mM MDC for 1hour at 37°C

Upstream tips
MDC is a fluorescent compound that stains autophagic vacuoles.
Protocol tips
Treat the cells with 0.05mM MDC for 1hour at 37°C

Publication protocol

MDC is a fluorescent compound that stains autophagic vacuoles. MRC5 cells were treated with 4 µM PAB and/or 2 mM 3MA for 36 h, then were incubated with 0.05 mM MDC at 37°C for 1 h. Following the incubation, cells were washed in PBS. Intracellular MDC was measured by fluorescence microscopy at an excitation wavelength of 380 nm and an emission filter of 525 nm (Leica Microsystems GmbH)

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