Beclin 1 Rabbit Polyclonal antibody

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Lyse cells using RIPA mixed with protease inhibitors and phosphatase inhibitors	- Dilute Primary Ab at 1:500-1:3000

Upstream tips
- Lyse cells using RIPA mixed with protease inhibitors and phosphatase inhibitors
Protocol tips
- Dilute Primary Ab at 1:500-1:3000

Publication protocol

A density of 1 × 105 A375 cells were seeded onto 6-well plates. After 24 h, cells were treated with varying concentrations of CP for 12 h, 24 h and 48 h. Then cells were washed twice immediately with pre-chilled PBS on ice. The cytoplasmic proteins were lysed using RIPA mixed with protease inhibitors and phosphatase inhibitors (Roche, Basel, Switzerland). The protein concentration was measured by the BCA protein assay kit (Fudebio, Hangzhou, China). The protocol we used was that same with our previous publication [38].

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