CYTO-ID® Autophagy detection kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Incase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time. - Incase of High CYTO-ID Green staining, it could be that the cell culture medium was depleted of nutrients and so change media 4-8 hours before the experiment.

Downstream tips
- Incase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time. - Incase of High CYTO-ID Green staining, it could be that the cell culture medium was depleted of nutrients and so change media 4-8 hours before the experiment.

Publication protocol

CYTO-ID Autophagy detection kit (Enzo Life Sciences, Lausen, Switzerland) is another commercially available tool to measure autophagy by FC. The probe used in this kit is a cationic amphiphilic tracer dye that primarily stains autophagosomes, while staining lysosomes minimally. This dye has been successfully used in both cell lines and primary cells from human and mouse origin (summarized in the company’s website). It can be used in conjunction with surface markers that discriminate different cell populations

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Reviews

Discussion

Papers

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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for CYTO-ID® Autophagy detection kit below.

Download manufacturer protocol

Videos

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