CYTO-ID® Autophagy detection kit

Protocol tips

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	Stain the cells with CYTO-ID for 30 min at 37°C in the dark

Protocol tips
Stain the cells with CYTO-ID for 30 min at 37°C in the dark

Publication protocol

For measurement of autophagy-associated intracellular vacuoles, we used the Cyto-ID Autophagy Detection Kit (Enzo Life Science, Loerrach, Germany) according to the manufacturers’ instructions. 5 × 105 CD4+ T-cells were activated in the presence or absence of CQ. Unstimulated cells and cells activated in drug-free medium (positive control) were included as controls. At the indicated time points, cells were harvested, washed once in staining buffer and were incubated with a cationic amphiphilic tracer dye for 20 minutes at room temperature. After washing incorporation of the dye into autophagic vacuoles was quantified by flow cytometry.

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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for CYTO-ID® Autophagy detection kit below.

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