LC3B Antibody Kit for Autophagy

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Lye cells in buffer containing RIPA and phenylmethylsulfonyl fluoride	Dilute the LC3B rabbit polyclonal antibody in blocking buffer to prepare 0.5 μg/mL working solution

Upstream tips
Lye cells in buffer containing RIPA and phenylmethylsulfonyl fluoride
Protocol tips
Dilute the LC3B rabbit polyclonal antibody in blocking buffer to prepare 0.5 μg/mL working solution

Publication protocol

The samples were homogenized in buffer containing RIPA and phenylmethylsulfonyl fluoride. The homogenates were assayed on polyacrylamide gels, transferred onto PVDF membranes (Thermo Fisher Scientific, Billerica, USA) and then probed with specific primary antibodies.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for LC3B Antibody Kit for Autophagy below.

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