MAP LC3α/β Antibody (N-20): sc-16755

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	For Western Blot use starting dilution 1:100, (dilution range 1:100-1:1000)	- It is more specific, provides a stronger signal and eliminates lot to lot variation characteristic of most, if not all, polyclonal antibodies.

Protocol tips
For Western Blot use starting dilution 1:100, (dilution range 1:100-1:1000)
Downstream tips
- It is more specific, provides a stronger signal and eliminates lot to lot variation characteristic of most, if not all, polyclonal antibodies.

Publication protocol

Immunofluorescence microscopy
Immunofluorescence microscopy was performed using standard methods as previously described [6]. Cover slips were analyzed using a fluorescence microscope (Leica DMRE, Leica Microsystems GmbH, Wetzlar, Germany). Deconvolution studies and 3-dimensional projections were performed using a DeltaVision system (Applied Precision, Issaquah, WA) with an Olympus IX-71microscope.

Immunoblotting analysis
Western blotting was performed using a standard protocol [7] and the Immun Star HRP detection kit (Bio-Rad Laboratories Inc., Hercules, CA, USA).

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Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for MAP LC3α/β Antibody (N-20): sc-16755 below.

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