LC3 Antibody (APG8B) (N-term)

Protocol tips

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This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LC3.	Dilute primary Ab at 1:100

Upstream tips
This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human LC3.
Protocol tips
Dilute primary Ab at 1:100

Publication protocol

Cells were fixed in 4% paraformaldehyde and incubated with anti-LC3 (Abgent, Oxfordshire, UK) or anti-caspase 3 (Cell Signaling, Danvers, MA, USA) and AlexaFluor-488 secondary antibody with standard washing procedures (Invitrogen, Paisley, UK). Analysis was carried out using an Olympus Fluorescence (Southend-on-Sea, Essex, UK) microscope.

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Manufacturer protocol

Download the product protocol from Abgent for LC3 Antibody (APG8B) (N-term) below.

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