CYTO-ID® Autophagy detection kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Cell density should not exceed 1x10 6/mL.	- Make a dye stain solution by diluting 1 µL CYTO-ID® Green Detection Reagent to 1 mL 1X Assay Buffer or cell culture medium without Phenol Red Indicator, supplemented with 5% FBS

Upstream tips
- Cell density should not exceed 1x10 6/mL.
Protocol tips
- Make a dye stain solution by diluting 1 µL CYTO-ID® Green Detection Reagent to 1 mL 1X Assay Buffer or cell culture medium without Phenol Red Indicator, supplemented with 5% FBS

Publication protocol

The flow cytometric investigation of Cyto-ID Green Detection Reagent stained cells was performed according to the manufacturer's protocol (Cyto-ID Autophagy Detection Kit, Enzo Life Sciences). Cells were analyzed using FACSCanto II (Becton Dickinson) as discussed previously [11]. Relative autophagy level was normalized to untreated control cells.

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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for CYTO-ID® Autophagy detection kit below.

Download manufacturer protocol

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