CYTO-ID® Autophagy detection kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
50nM Rapamycin treated cells can be used as positive control.	Incubate cells for 30 minutes at 37°C in the dark, wash with 1× assay buffer, and resuspend in 500 μL fresh 1× assay buffer. Subject the cells to flow cytometric analysis using the green (FL1) channel.

Upstream tips
50nM Rapamycin treated cells can be used as positive control.
Protocol tips
Incubate cells for 30 minutes at 37°C in the dark, wash with 1× assay buffer, and resuspend in 500 μL fresh 1× assay buffer. Subject the cells to flow cytometric analysis using the green (FL1) channel.

Publication protocol

Ramos and Daudi cells were treated with 40 ng/mL of Rituximab-MMAE for 24 h. Afterward, the cells were stained by Cyto-ID Autophagy Detection Kit (ENZO Life Science, Farmingdale, NY, USA) following the manufacturer’s instruction. Cells were then incubated with autophagy inducer, rapamycin (50 nM) for positive control and observed by inverted confocal microscope (Carl Zeiss LSM710, Carl Zeiss, Germany).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Autophagy assay cell type - Ramos using CYTO-ID® Autophagy detection kit from Enzo Life Sciences.

Manufacturer protocol

Download the product protocol from Enzo Life Sciences for CYTO-ID® Autophagy detection kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Autophagy assay cell type - Ramos using CYTO-ID® Autophagy detection kit from Enzo Life Sciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.