LC3B Mouse Monoclonal Antibody (9H5)

Protocol tips

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	The lysate was centrifuged at 12,000 rpm for 10 min at 4°C. Equivalent amount protein in each sample was separated in SDS-PAGE gel and electro-transferred onto polyvinylidene fluoride membranes. PVDF membranes were incubated with primary antibody and second antibody, respectively. Immobilon™ Western Chemiluminescent HRP Substrate (Millipore, Billerica, MA, USA) was used to visualize the immunoreactive bands, and densitometric values were quantified by ImageJ 1.47v (National Institutes of Health, USA).

Protocol tips

The lysate was centrifuged at 12,000 rpm for 10 min at 4°C. Equivalent amount protein in each sample was separated in SDS-PAGE gel and electro-transferred onto polyvinylidene fluoride membranes. PVDF membranes were incubated with primary antibody and second antibody, respectively. Immobilon™ Western Chemiluminescent HRP Substrate (Millipore, Billerica, MA, USA) was used to visualize the immunoreactive bands, and densitometric values were quantified by ImageJ 1.47v (National Institutes of Health, USA).

Publication protocol

The lysate was centrifuged at 12,000 rpm for 10 min at 4°C. Equivalent amount protein in each sample was separated in SDS-PAGE gel and electro-transferred onto polyvinylidene fluoride membranes. PVDF membranes were incubated with primary antibody and second antibody, respectively. Immobilon™ Western Chemiluminescent HRP Substrate (Millipore, Billerica, MA, USA) was used to visualize the immunoreactive bands, and densitometric values were quantified by ImageJ 1.47v (National Institutes of Health, USA).

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