CYTO-ID® Autophagy detection kit

Autophagy assay cell type - H1299

Experiment
Autophagy assay cell type - H1299
Product
CYTO-ID® Autophagy detection kit from Enzo Life Sciences
Manufacturer
Enzo Life Sciences

Protocol tips

Upstream tips
This kit uses a green fluorescent probe that reacts with autophagic vacuoles and is detectable by flow cytometry
Protocol tips
- 2 µL of CYTO-ID® Green Detection Reagent and 1 µL of Hoechst 33342 Nuclear Stain for every 1 mL of 1X Assay Buffer or complete cell growth medium
Downstream tips
Incase of Low CYTO-ID Green dye staining in all treatments, increase the reagent concentration (500X dilution of the dye is recommended) and the incubation time.

Incase of High CYTO-ID Green staining, it could be that the cell culture medium was depleted of nutrients and so change media 4-8 hours before the experiment.

Publication protocol

Autophagy assays were performed using a Cyto-ID Autophagy Detection Kit (Enzo Life Sciences, Farmingdale, NY) using the protocol described by the manufacturer. Live cells were analyzed by fluorescent microscopy using an EVOS FL imaging system (Life Technologies). Corrected total cell fluorescence was calculated as described in [68] using ImageJ software.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Autophagy assay cell type - H1299 using CYTO-ID® Autophagy detection kit from Enzo Life Sciences.

Paper title
Rheb may complex with RASSF1A to coordinate Hippo and TOR signaling
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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for CYTO-ID® Autophagy detection kit below.

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