ATG5 Antibody Novus

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Dilute the rabbit anti-LC3B primary antibody (NB100-2220) (~2 ug/mL) in blocking buffer and incubate the membrane for 1 hour at room temperature

Protocol tips
Dilute the rabbit anti-LC3B primary antibody (NB100-2220) (~2 ug/mL) in blocking buffer and incubate the membrane for 1 hour at room temperature

Publication protocol

The STAT3 pathway was detected as previously described (26). Total harvested cells were lysed to perform immunoblots as previously reported (27). Immunoblotting was subjected to semi-quantitative analysis using ImageJ software.

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Manufacturer protocol

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