LC3B (D11) XP® Rabbit mAb (Biotinylated)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Lyse cells in lysis buffer (1% triton-X, 0.1% SDS, 50 mM HEPES pH 7.4, 150 mM NaCl, 100 mM NaF, 10 mM EDTA, 10 mM Na4P2O7 containing protease inhibitor cocktail )	Dilute primary Ab at 1:1000

Upstream tips
Lyse cells in lysis buffer (1% triton-X, 0.1% SDS, 50 mM HEPES pH 7.4, 150 mM NaCl, 100 mM NaF, 10 mM EDTA, 10 mM Na4P2O7 containing protease inhibitor cocktail )
Protocol tips
Dilute primary Ab at 1:1000

Publication protocol

Western blotting.
Cell lysates were prepared in lysis buffer (1% triton-X, 0.1% SDS, 50 mM HEPES pH 7.4, 150 mM NaCl, 100 mM NaF, 10 mM EDTA, 10 mM Na4P2O7 containing protease inhibitor cocktail from Roche) and transferred to nitrocellulose or immobulin-P membranes as previously described in reference 31. Membranes were probed using standard immunoblotting techniques. ATG12 and LC3B antibodies were from Cell Signaling, and the Erk2 antibody was a kind gift from Prof. Chris Marshall (Ab122, CJ Marshall, Institute of Cancer Research).


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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for LC3B (D11) XP® Rabbit mAb (Biotinylated) below.

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