PureLink™ Quick Gel Extraction Kit and PCR Purification Combo Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
The amount of agarose excised from the gel should be as small as possible.	Remove the flow-through by aspiration. Avoid contamination of the collection tube rim.

Upstream tips
The amount of agarose excised from the gel should be as small as possible.
Protocol tips
Remove the flow-through by aspiration. Avoid contamination of the collection tube rim.

Publication protocol

The DNA amplified by MS‐HRM was submitted to a DNA purification methodology, following the protocol from PureLink Quick Gel Extraction kit and PCR Purification Combo Kit (Invitrogen, USA). The purified DNA amplificons were submitted to a Bisulfite Sequencing. BigDye V. 3.1 (ThermoScientific) was used for Bisulfite genomic Sanger sequencing procedure on an ABI 3730 capillary sequencer (ThermoScientific). Sequences were compared to the reference genome (Genbank accession number: NG_01295) using Blast tools to assess the sequence identity. The software M‐Coffee tool was used to confirm the methylated and unmethylated cytosines present in the bisulfite‐treated DNA sequence.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA gel extraction / PCR product purification Product size < 15Kb using PureLink™ Quick Gel Extraction Kit and PCR Purification Combo Kit from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for PureLink™ Quick Gel Extraction Kit and PCR Purification Combo Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA gel extraction / PCR product purification Product size < 15Kb using PureLink™ Quick Gel Extraction Kit and PCR Purification Combo Kit from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.