Anti-LC3B antibody produced in rabbit

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Antibody is recommended for western blotting and immunofluorescence staining	Methyl-beta cyclodextrin treatment in breast cancer cell lines induces autophagic flux that can be detected with Anti -LC3B antibody by western blot.

Upstream tips
Antibody is recommended for western blotting and immunofluorescence staining
Protocol tips
Methyl-beta cyclodextrin treatment in breast cancer cell lines induces autophagic flux that can be detected with Anti -LC3B antibody by western blot.

Publication protocol

After the designated treatments, Laemmli SDS buffer were used to lyse the collected samples (62.5 mM Tris, pH 6.8, 25% glycerol, 2% SDS [Vivantis, PB0640], phosphatase inhibitor [Thermo Scientific, 78428] and proteinase inhibitor cocktail [Roche, 11697498001]). An equal amount of protein of each sample was resolved by SDS-PAGE, and then the SDS-PAGE gels were transferred onto PVDF membrane (Bio-Rad, 162–0177). Membranes were blocked for 30 min with blocking buffer (Thermo, 37538) and followed by incubations with the indicated primary and secondary antibodies. Then the membranes were visualized with the enhanced chemiluminescence method (Thermo Scientific, 34076) by the ImageQuant LAS 500 (GE).

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