CYTO-ID® Autophagy detection kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Staining for 20 min at 37degreec C.

Protocol tips
Staining for 20 min at 37degreec C.

Publication protocol

Autophagic vacuoles were analyzed using a Cyto-ID autophagy detection kit (Enzo Life Sciences) according to the manufacturer’s protocol. In brief, cells were stained with Cyto-ID autophagy detection dye and Hoechst 33342 (Life Technologies) for 20 min at 37°C. Images were taken under the microscope (Nikon). EM analysis of autophagy was performed as described previously (Zhao et al., 2012).

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Manufacturer protocol

Download the product protocol from Enzo Life Sciences for CYTO-ID® Autophagy detection kit below.

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