LC3B Antibody

Protocol tips

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	Western analysis and cytokine ELISAs (for TNF-α and IL-6) were performed, as described previously (17). NF-κB luciferase assays were also performed, as described previously (17). Briefly, cells were cotransfected with a NF-κB–luc construct and siRNA (for HaCaT cells) or transduced with Ad5HSV-NF-κB-luc. Following stimulation with MALP-2 (100 ng/ml) for 24 h, cells were lysed and their luciferase activity was analyzed using the Luciferase Assay System (Promega, Madison, WI), according to the manufacturer’s instructions.

Protocol tips

Western analysis and cytokine ELISAs (for TNF-α and IL-6) were performed, as described previously (17). NF-κB luciferase assays were also performed, as described previously (17). Briefly, cells were cotransfected with a NF-κB–luc construct and siRNA (for HaCaT cells) or transduced with Ad5HSV-NF-κB-luc. Following stimulation with MALP-2 (100 ng/ml) for 24 h, cells were lysed and their luciferase activity was analyzed using the Luciferase Assay System (Promega, Madison, WI), according to the manufacturer’s instructions.

Publication protocol

Western analysis and cytokine ELISAs (for TNF-α and IL-6) were performed, as described previously (17). NF-κB luciferase assays were also performed, as described previously (17). Briefly, cells were cotransfected with a NF-κB–luc construct and siRNA (for HaCaT cells) or transduced with Ad5HSV-NF-κB-luc. Following stimulation with MALP-2 (100 ng/ml) for 24 h, cells were lysed and their luciferase activity was analyzed using the Luciferase Assay System (Promega, Madison, WI), according to the manufacturer’s instructions.

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Manufacturer protocol

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