SQSTM1 Antibody (H-290)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000)

Protocol tips
Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000)

Publication protocol

Western analysis and cytokine ELISAs (for TNF-α and IL-6) were performed, as described previously (17). NF-κB luciferase assays were also performed, as described previously (17). Briefly, cells were cotransfected with a NF-κB–luc construct and siRNA (for HaCaT cells) or transduced with Ad5HSV-NF-κB-luc. Following stimulation with MALP-2 (100 ng/ml) for 24 h, cells were lysed and their luciferase activity was analyzed using the Luciferase Assay System (Promega, Madison, WI), according to the manufacturer’s instructions.

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Manufacturer protocol

Download the product protocol from Santa Cruz Biotechnology for SQSTM1 Antibody (H-290) below.

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