CYTO-ID® Autophagy detection kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Treat cells with Cyto-ID and Hoechst 33,342 at 37 °C for 30 min. - Wash cells with 1X assay buffer and immediately analyz

Protocol tips
- Treat cells with Cyto-ID and Hoechst 33,342 at 37 °C for 30 min. - Wash cells with 1X assay buffer and immediately analyz

Publication protocol

H9C2 cells were seeded into clear bottom imaging tissue culture plates (NEST Biotechnology Co., Ltd., Jiangsu, China). After 24 h of incubation, cells were treated with an equal volume of the vehicle with or without XFZY (18, 9, or 4.5 mg/mL) for another H10/R2 incubation. Cells were treated with Cyto-ID Autophagy Detection Kit (Enzo Life Sciences, Inc.) according to the manufacturer’s instruction. Briefly, cells were washed twice with assay buffer and stained with Cyto-ID and Hoechst 33,342 at 37 °C for 30 min. After incubation, cells were washed again with 1 × assay buffer and immediately analyzed with an Olympus fluorescence microscope.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Autophagy assay cell type - H9C2 using CYTO-ID® Autophagy detection kit from Enzo Life Sciences.

Manufacturer protocol

Download the product protocol from Enzo Life Sciences for CYTO-ID® Autophagy detection kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Autophagy assay cell type - H9C2 using CYTO-ID® Autophagy detection kit from Enzo Life Sciences. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.