Anti-LC3B antibody produced in rabbit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- RIPA buffer is composed of 1 × PBS, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM phenyl methylsulfonyl fluoride, and protease inhibitors for better results

Upstream tips
- RIPA buffer is composed of 1 × PBS, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM phenyl methylsulfonyl fluoride, and protease inhibitors for better results

Publication protocol

Cell extracts for Western blotting were prepared in RIPA buffer (1 × PBS, 1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM phenyl methylsulfonyl fluoride, and protease inhibitors). Lysates were separated by SDS-PAGE and were transferred to PVDF membranes (Millipore, Billerica, MA); the immune complex was detected by chemiluminescence (GE Healthcare, Wauwatosa, WI).

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Manufacturer protocol

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