BECN1 Antibody (H-300)

Protocol tips

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	- Incubate the membranes with primary antibodies at 4C.

Protocol tips
- Incubate the membranes with primary antibodies at 4C.

Publication protocol

After treatment, cell lysates were harvested and then subjected to electrophoresis on 7–12% SDS-PAGE gels. Fractionated proteins were electrophoretically transferred onto PVDF membranes. The incubation of the membranes with primary antibodies (anti-caspase-3, anti-caspase-8, anti-PARP, anti-Bcl-2, anti-LC3, anti-beclin-1, anti-mTOR, anti-PI3K, anti-Akt and anti-ERK1/2) was carried out at 4°C. The incubation of the membranes with horseradish peroxidase (HRP)-conjugated secondary antibodies was carried out at room temperature for 1 h, and proteins detected by enhanced chemiluminescence reagents (Pierce Biotechnology, Rockford, IL, USA), as suggested by the manufacturer. After normalization to actin, the control sample was assigned an arbitrary value of 1, and the fold change, in response to resveratrol treatment, was calculated.

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Manufacturer protocol

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