Beclin-1 Antibody

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	- Dilute primary ab 1:1000 and incubate at overnight at 4°C

Protocol tips
- Dilute primary ab 1:1000 and incubate at overnight at 4°C

Publication protocol

Cells were lysed in RIPA buffer (Beyotime, P0013C), and total protein concentration was measured using a bicinchoninic acid protein assay kit (Tiangen Biotech, PA115). Proteins from whole cell extracts were resolved using denaturing SDS-PAGE and analyzed by western blotting. Antibodies used in western blotting analyses are listed in Table 2. Immunoblots shown in the accompanying figures are derived from 3 independent experiments. Intensities of protein bands were quantified by densitometry using the software ImageJ (http://imagej.nih.gov/ij/).

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Papers

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Manufacturer protocol

Download the product protocol from Cell Signaling Technology for Beclin-1 Antibody below.

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Videos

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