DMEM–Dulbecco's Modified Eagle Medium

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	supplemented with 10% fetal bovine serum (ThermoFisher Scientific), 1% penicillin/streptomycin, 1% non-essential amino acids, 1% L-glutamine and 1% sodium pyruvate in a 5% CO2 atmosphere at 37°C.

Protocol tips
supplemented with 10% fetal bovine serum (ThermoFisher Scientific), 1% penicillin/streptomycin, 1% non-essential amino acids, 1% L-glutamine and 1% sodium pyruvate in a 5% CO2 atmosphere at 37°C.

Publication protocol

HEK-293T (Friedemann Weber, University of Gießen) and MA-104 (Friedemann Weber, University of Gießen), Vero (Jindrich Cinatl, University of Frankfurt), Vero E6 (ATCC, ATCC CRL-1586), Calu-3 (ATCC HTB-55) and RhiLu-hACE2 (provided by author Marcel A. Müller [56]) cells were cultivated in Dulbecco’s modified Eagles medium (DMEM) supplemented with 10% fetal bovine serum (ThermoFisher Scientific), 1% penicillin/streptomycin, 1% non-essential amino acids, 1% L-glutamine and 1% sodium pyruvate in a 5% CO2 atmosphere at 37°C. HAE were generated and maintained. RVFV Cl 13 was a kind gift from Friedemann Weber (University of Gießen). Infection experiments with rSCV were done under biosafety level 3 conditions with enhanced respiratory personal protection equipment.

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