Minimum Essential Media (MEM)

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	enriched with fetal bovine serum at 1%, 25 µg/L of gentamicin, and 200 mmol/L of L-glutamine (maintenance medium)

Protocol tips
enriched with fetal bovine serum at 1%, 25 µg/L of gentamicin, and 200 mmol/L of L-glutamine (maintenance medium)

Publication protocol

The MDCK cell lines were obtained from ATCC (American Type Culture Collection). The cells were culture in an essential culture medium with Earle's salts (Gibco BRL, Grand Island, NY), enriched with fetal bovine serum at 10%, 25 µg/L of gentamicin, and 200 mmol/L of L-glutamine (growth medium). The infected cells were stored in a minimum essential medium with fetal bovine serum at 1%, 25 µg/L of gentamicin, and 200 mmol/L of L-glutamine (maintenance medium). All the cells were cultured at 37°C in a humidified atmosphere with 5% of CO2 and 95% air.

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