EZ-10 Spin Column PCR Product Purification kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
The amount of agarose excised from the gel should be as small as possible.	Remove the flow-through by aspiration. Avoid contamination of the collection tube rim.

Upstream tips
The amount of agarose excised from the gel should be as small as possible.
Protocol tips
Remove the flow-through by aspiration. Avoid contamination of the collection tube rim.

Publication protocol

MTHFR 677C>T genotyping:
The 320-bp fragment of MTHFR containing the 677C>T polymorphism was
amplified by polymerase chain reaction (PCR) using the following primers: forward
5'-AAGCAGAGGACTCTCTCTGCCC-3' and reverse 5'-CCCCCAGCCTGTGCGAGGACGGT-3',
designed in our laboratory and with an annealing temperature of 57°C for 35 cycles. The fragments
obtained were purified using the EZ-10 Spin Column PCR Product Purification kit (Bio Basic,
Markham, ON, Canada) following manufacturer instructions and sequenced using an ABI3130
automatic sequencer (Life Technologies, Carlsbad, CA, USA). Sequences were aligned using the
BioEdit v7.0.5 software (Hall, 1999).
TSER genotyping:
The fragment with 28 bp in tandem (TSER) was amplified by PCR using the primers and
conditions described by Ulrich et al. (2002). Polymorphic bands of 215 (2R) and 243 (3R) were
confirmed after 14% polyacrylamide gel electrophoresis and silver staining. To analyze the C/G
SNP, samples with 3R alleles were purified using the EZ-10 Spin Column PCR Product Purification
kit (Bio Basic), followed by digestion with HaeIII (Invitrogen, Carlsbad, CA, USA) and visualized by
silver staining after 12% polyacrylamide gel electrophoresis.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA gel extraction / PCR product purification Product size > 15Kb using EZ-10 Spin Column PCR Product Purification kit from Bio Basic.

Manufacturer protocol

Download the product protocol from Bio Basic for EZ-10 Spin Column PCR Product Purification kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA gel extraction / PCR product purification Product size > 15Kb using EZ-10 Spin Column PCR Product Purification kit from Bio Basic. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.