MEM (Eagle) Liquid Medium

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	MDCK II cells were maintained in Earle’s minimum essential medium supplemented with 4 mM glutamine, 0.2 mg/mL of both penicillin and streptomycin (Biochrom, Berlin, Germany), 10% (v/v) fetal calf serum (PAA, Pasching, Austria) in a 5% CO2 humidified incubator (HERA cell 150, Heraeus, Germany). Cells were subcultured weekly after reaching confluence by washing with PBS, followed by trypsinization and centrifugation at 110g.

Protocol tips

MDCK II cells were maintained in Earle’s minimum essential medium supplemented with 4 mM glutamine, 0.2 mg/mL of both penicillin and streptomycin (Biochrom, Berlin, Germany), 10% (v/v) fetal calf serum (PAA, Pasching, Austria) in a 5% CO2 humidified incubator (HERA cell 150, Heraeus, Germany). Cells were subcultured weekly after reaching confluence by washing with PBS, followed by trypsinization and centrifugation at 110g.

Publication protocol

MDCK II cells were maintained in Earle’s minimum essential medium supplemented with 4 mM glutamine, 0.2 mg/mL of both penicillin and streptomycin (Biochrom, Berlin, Germany), 10% (v/v) fetal calf serum (PAA, Pasching, Austria) in a 5% CO2 humidified incubator (HERA cell 150, Heraeus, Germany). Cells were subcultured weekly after reaching confluence by washing with PBS, followed by trypsinization and centrifugation at 110g.

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