Minimum Essential Media (MEM)

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	supplemented with 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA), 1% penicillin-streptomycin combination, 1% glutamine, and 2% NaHCO3 (pH 7.4) at 37 °C in a 5% CO2 atmosphere.

Protocol tips
supplemented with 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA), 1% penicillin-streptomycin combination, 1% glutamine, and 2% NaHCO3 (pH 7.4) at 37 °C in a 5% CO2 atmosphere.

Publication protocol

Baby hamster kidney (BHK-21) cells were stored in the laboratory. The cells were cultured in minimal essential medium (MEM; Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA), 1% penicillin-streptomycin combination, 1% glutamine, and 2% NaHCO3 (pH 7.4) at 37 °C in a 5% CO2 atmosphere.

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