Dulbecco’s Modified Eagle’s Medium - high glucose

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	with 10% fetal calf serum (Sigma-Aldrich, St. Louis, MO) and 1% antibiotic-antimitotic solution (Sigma-Aldrich, St. Louis, MO)

Protocol tips
with 10% fetal calf serum (Sigma-Aldrich, St. Louis, MO) and 1% antibiotic-antimitotic solution (Sigma-Aldrich, St. Louis, MO)

Publication protocol

For liquid phase exposure, the particulate matter (PM) emissions were first collected on a 47 mm Teflon filter (Sigma-Aldrich, St. Louis, MO) as illustrated in Fig. 2B. To extract the collected PM, the filter was submerged in 100 ml of deionized water and sonicated for 30 minutes. This extract was then further diluted to different strengths with Dulbecco’s modified Eagle’s medium (DMEM F-12) (Sigma-Aldrich, St. Louis, MO) with 10% fetal calf serum (Sigma-Aldrich, St. Louis, MO) and 1% antibiotic-antimitotic solution (Sigma-Aldrich, St. Louis, MO). Particle suspensions (1000-200-50-20-10 µg/ml, denoted in the rest of the study as dilution ratios of 1:1, 1:5, 1:20, 1:50, 1:100, respectively) were sonicated with a Branson sonicator bath (Hach, Loveland, CO) for 30 seconds immediately before being added to cell cultures. The cell media was removed prior to the applewood extract exposure in the liquid phase. The volume of applewood extracts was verified to be 150 μl in each well.

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Dulbecco’s Modified Eagle’s Medium - high glucose below.

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