Gibco™ RPMI 1640 Medium

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	supplemented with 10% fetal bovine serum (GIBCO-BRL, Life Technologies).

Protocol tips
supplemented with 10% fetal bovine serum (GIBCO-BRL, Life Technologies).

Publication protocol

The human HNSCC cell line A253 was purchased from American Type Culture Collection (Rockville, MD, USA) and grown as a monolayer culture in RPMI 1640 medium supplemented with 10% fetal bovine serum (GIBCO-BRL, Life Technologies). All treatments were carried out using exponentially growing cell cultures. The cell lines were free from mycoplasma as tested with the GEN-PROBE mycoplasma TC rapid detection system every 2 months (GEN-PROBE Inc, San Diego, CA, USA)

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