McCoy's 5A Medium, ATCC® 30-2007™

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	The A253 cell line was purchased from ATCC and grown in McCoy’s 5a media (also sourced from ATCC), per supplier’s guidance. Primary salivary hS/PCs were obtained as previously described (Pradhan-Bhatt et al. 2013, 2014; Srinivasan et al. 2017) and grown in Hepatocyte Defined media (Discovery Labware) containing epidermal growth factor at a concentration of 5 μg/500 mL. Cells in passages 5 to 7 were used for all experiments.

Protocol tips

The A253 cell line was purchased from ATCC and grown in McCoy’s 5a media (also sourced from ATCC), per supplier’s guidance. Primary salivary hS/PCs were obtained as previously described (Pradhan-Bhatt et al. 2013, 2014; Srinivasan et al. 2017) and grown in Hepatocyte Defined media (Discovery Labware) containing epidermal growth factor at a concentration of 5 μg/500 mL. Cells in passages 5 to 7 were used for all experiments.

Publication protocol

The A253 cell line was purchased from ATCC and grown in McCoy’s 5a media (also sourced from ATCC), per supplier’s guidance. Primary salivary hS/PCs were obtained as previously described (Pradhan-Bhatt et al. 2013, 2014; Srinivasan et al. 2017) and grown in Hepatocyte Defined media (Discovery Labware) containing epidermal growth factor at a concentration of 5 μg/500 mL. Cells in passages 5 to 7 were used for all experiments.

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