DMEM–Dulbecco's Modified Eagle Medium

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	DMEM medium (Gibco, USA) containing 10% fetal bovine serum (FBS, Gibco, USA). A2780/DPP was alternately fed with medium containing 9 μg/mL cisplatin to maintain drug-resistance and further cultured in drug-free medium for one week before follow-up experiments.

Protocol tips
DMEM medium (Gibco, USA) containing 10% fetal bovine serum (FBS, Gibco, USA). A2780/DPP was alternately fed with medium containing 9 μg/mL cisplatin to maintain drug-resistance and further cultured in drug-free medium for one week before follow-up experiments.

Publication protocol

The human ovarian cancer cell line A2780 and its cisplatin-resistant subline A2780/DDP were cultured in DMEM medium (Gibco, USA) containing 10% fetal bovine serum (FBS, Gibco, USA), in a humidified incubator with 5% CO2 at 37°C. A2780/DPP was alternately fed with medium containing 9 μg/mL cisplatin to maintain drug-resistance and further cultured in drug-free medium for one week before follow-up experiments. Both cell lines were obtained and preserved in Gynecological Oncology of Biotherapy Laboratory, West China Second University Hospital, Sichuan University, Chengdu, China.

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