DMEM–Dulbecco's Modified Eagle Medium

Mammalian cell culture media A2780

Experiment
Mammalian cell culture media A2780
Product
DMEM–Dulbecco's Modified Eagle Medium from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
DMEM medium (Gibco, USA) containing 10% fetal bovine serum (FBS, Gibco, USA). A2780/DPP was alternately fed with medium containing 9 μg/mL cisplatin to maintain drug-resistance and further cultured in drug-free medium for one week before follow-up experiments.

Publication protocol

The human ovarian cancer cell line A2780 and its cisplatin-resistant subline A2780/DDP were cultured in DMEM medium (Gibco, USA) containing 10% fetal bovine serum (FBS, Gibco, USA), in a humidified incubator with 5% CO2 at 37°C. A2780/DPP was alternately fed with medium containing 9 μg/mL cisplatin to maintain drug-resistance and further cultured in drug-free medium for one week before follow-up experiments. Both cell lines were obtained and preserved in Gynecological Oncology of Biotherapy Laboratory, West China Second University Hospital, Sichuan University, Chengdu, China.

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Papers

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Paper title
MiR-130a and MiR-374a Function as Novel Regulators of Cisplatin Resistance in Human Ovarian Cancer A2780 Cells
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for DMEM–Dulbecco's Modified Eagle Medium below.

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