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RPMI 1640 medium containing 10% FCS, 1% penicillin/streptomycin (PAN Biotech, Aidenbach, Germany) and 1.5% L-glutamine. |
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| Protocol tips |
| RPMI 1640 medium containing 10% FCS, 1% penicillin/streptomycin (PAN Biotech, Aidenbach, Germany) and 1.5% L-glutamine. |
Publication protocol
A2780 (No. 93112519) and the cisplatin resistant A2780cis (No. 93112517) human ovarian carcinoma cell lines were purchased from the ECACC, UK and cultivated by 37°C and 5% CO2 in RPMI 1640 medium containing 10% FCS, 1% penicillin/streptomycin (PAN Biotech, Aidenbach, Germany) and 1.5% L-glutamine. Upon receipt, cells were directly frozen in aliquots (master cell bank) from which they were cultivated for a maximum of ten passages for functional studies and gene expression profiling. Authenticity of cells was confirmed by short tandem repeat (STR) profiling. The maintenance of cisplatin resistance in A2780cis cells as well as the absence of mycoplasma in cell culture was confirmed every second week.
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Papers
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Paper title
Heparin antagonizes cisplatin resistance of A2780 ovarian cancer cells by affecting the Wnt signaling pathway
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