Gibco™ RPMI 1640 Medium

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	supplemented with 5% heat-inactivated fetal bovine serum (Hyclone, South Logan, UT, USA), 2 mm glutamine and antibiotics (100 U ml−1 penicillin and 100 μg ml−1 streptomycin)

Protocol tips
supplemented with 5% heat-inactivated fetal bovine serum (Hyclone, South Logan, UT, USA), 2 mm glutamine and antibiotics (100 U ml−1 penicillin and 100 μg ml−1 streptomycin)

Publication protocol

The human NSCLC cell lines5 A549, H460, PC-9 and HCC827 were maintained in RPMI 1640 medium (Gibco, Grand Island, NY, USA) supplemented with 5% heat-inactivated fetal bovine serum (Hyclone, South Logan, UT, USA), 2 mm glutamine and antibiotics (100 U ml−1 penicillin and 100 μg ml−1 streptomycin) at 37 °C in a humidified atmosphere of 5% CO2. The A549 (GR, CR, PR, GemR and IR),5 PC-9 (GR, CR, ER, CLR and ZDR), H460 (CR, PR and IR) and HCC827 (GR, CR, PR, ER and CLR) cell lines have been established in previous studies (GR, gefitinib resistant; CR, cisplatin resistant; CLR, CL-387 and 785 resistant; PR, paclitaxel resistant; ZDR, ZD6474 resistant; ER, erlotinib resistant; GemR, gemcitabine resistant and IR, irradiation resistant).14 The A549/GR-shPrx II cell lines were established by the transfection of A549/GR cells with shPrx II-1, shPrx II-2, shPrx II-3 and scrambled short hairpin RNA.

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