DMEM–Dulbecco's Modified Eagle Medium

Mammalian cell culture media A549

Experiment
Mammalian cell culture media A549
Product
DMEM–Dulbecco's Modified Eagle Medium from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Protocol tips
supplemented with 10% fetal bovine serum (FBS) and penicillin (100 U/mL)/streptomycin (100 U/mL)

Publication protocol

The human lung cancer A549 cell line was purchased from the Type Culture Collection of the Chinese Academy of Sciences, Shanghai, China. A549 cells were obtained from a 58-year-old white male with poorly differentiated lung adenocarcinoma. A549/PTX and A549/DDP cells were purchased from Shanghai MEIXUAN Biological Science and Technology Co, Ltd. A549/PTX and A549/DDP cells were cultured in RPMI-1640, and A549 cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and penicillin (100 U/mL)/streptomycin (100 U/mL) at 37 °C in a humidified atmosphere with 5% CO2.

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Papers

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Paper title
Cathepsin L upregulation-induced EMT phenotype is associated with the acquisition of cisplatin or paclitaxel resistance in A549 cells
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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for DMEM–Dulbecco's Modified Eagle Medium below.

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