DMEM–Dulbecco's Modified Eagle Medium

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	supplemented with 10% fetal bovine serum (FBS) and penicillin (100 U/mL)/streptomycin (100 U/mL)

Protocol tips
supplemented with 10% fetal bovine serum (FBS) and penicillin (100 U/mL)/streptomycin (100 U/mL)

Publication protocol

The human lung cancer A549 cell line was purchased from the Type Culture Collection of the Chinese Academy of Sciences, Shanghai, China. A549 cells were obtained from a 58-year-old white male with poorly differentiated lung adenocarcinoma. A549/PTX and A549/DDP cells were purchased from Shanghai MEIXUAN Biological Science and Technology Co, Ltd. A549/PTX and A549/DDP cells were cultured in RPMI-1640, and A549 cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and penicillin (100 U/mL)/streptomycin (100 U/mL) at 37 °C in a humidified atmosphere with 5% CO2.

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