RPMI 1640

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	supplemented with 10% FCS

Protocol tips
supplemented with 10% FCS

Publication protocol

Peripheral blood lymphocytes of healthy donors were isolated from buffy coats on Histopaque 1077 (Sigma-Aldrich) as described earlier [35]. Leukemia cell line CML-T1 (German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany) and HL-60 (European Collection of Animal Cell Cultures, Great Britain) were cultivated in RPMI 1640 (Biochrom AG, Germany) supplemented with 10% FCS, 37°C, and 5% CO2 atmosphere. Decitabine, ATRA (both from Sigma-Aldrich), and SAHA (Cayman Chemical Company, Ann Arbor, MI, USA) were added from 1–100 mM stock solution to final concentration of 1 μM for 48 h. N-acetyl-L-cysteine (NAC, Sigma-Aldrich) and caspase inhibitors z-VAD-fmk (Santa Cruz) and Q-VD-OPh (R&D Systems) were added to final concentration of 10 mM (NAC), 50 μM (z-VAD-fmk), and 10–30 μM (Q-VD-OPh), respectively.

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Manufacturer protocol

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