Gibco™ DMEM, high glucose

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	supplemented with 10% FBS, 1% penicillin/streptomycin, and were growth at 37 °C in a humidified atmosphere consisting of 5% CO2.

Protocol tips
supplemented with 10% FBS, 1% penicillin/streptomycin, and were growth at 37 °C in a humidified atmosphere consisting of 5% CO2.

Publication protocol

Human pancreatic cancer PANC-1, BxPC-3 cells were kindly provided by Stem Cell Bank, Chinese Academy of Science (SCSP-535, TCHu 12, China). Human normal prancreatic duct hTERT-HPNE cells were provided by Cobioer (CBP60875, China). PANC-1, BxPC-3 and hTERT-HPNE cells were authenticated by short tandem repeat profiling at the Stem Cell Bank, Chinese Academy of Science, and comparison to the published profile.And PANC-1, BxPC-3 and hTERT-HPNE cells were last tested by short tandem repeat profiling on May and November 2018. These cells were cultured in DMEM (Gibco) or 1640 (Gibco) supplemented with 10% FBS, 1% penicillin/streptomycin, and were growth at 37 °C in a humidified atmosphere consisting of 5% CO2.

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